Acute Waterborne Nickel Toxicity in the Rainbow Trout (Oncorhynchus mykiss) Occurs by a Respiratory Rather Than Ionoregulatory Mechanism

The acute mechanism of toxicity of waterborne nickel (Ni) was investigated in the rainbow trout (Oncorhynchus mykiss) in moderately hard (∼140 mg l−1 as CaCO3) Lake Ontario water, where the 96-h LC50 for juvenile trout (1.5–3.5 g) was 15.3 mg (12.7–19.0, 95% C.L.) dissolved Ni l−1. No marked impact of Ni exposure on average unidirectional or net fluxes of Na+, Cl−, or Ca2+ was observed in juvenile trout exposed for 48–60 h to 15.6 mg Ni l−1 as NiSO4. Furthermore, when adult rainbow trout (200–340 g) were fitted with indwelling dorsal aortic catheters and exposed for 117 h to 11.6 mg Ni l−1 as NiSO4, plasma ions (Na+, Cl−, Ca2+, and Mg2+) were all well conserved. However, mean arterial oxygen tension dropped gradually to ∼35% of control values. This drop in PaO2 was accompanied by an acidosis primarily of respiratory origin. PaCO2 rose to more than double control values with a concomitant drop in arterial pH of 0.15 units. Acute respiratory toxicity was further evidenced by a significant increase in hematocrit (Ht), and plasma lactate, and a significant decrease in spleen hemoglobin (Hb). Following 117 h of exposure to 11.6 mg Ni l−1, the gill, intestine, plasma, kidney, stomach, and heart accumulated Ni significantly, with increases of 60, 34, 28, 11, 8, and 3-fold, respectively. Brain, white muscle, liver, and bile did not significantly accumulate Ni. Plasma Ni exhibited a remarkable linear increase with time to levels approximately 30-fold higher than controls. We conclude that in contrast to most other metals, Ni is primarily a respiratory, rather than an ionoregulatory, toxicant at exposure levels close to the 96-h LC50. The implications of a waterborne metal as an acute respiratory toxicant (as opposed to ionoregulatory toxicants such as Cu, Ag, Cd, or Zn) with respect to toxicity modeling are discussed.

Publisher - Elsevier

Subjects - Chemical, Nickel; Fish, Rainbow Trout

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Citation: Pane EF, Richards JG, Wood CM. 2003. Acute Waterborne Nickel Toxicity in the Rainbow Trout (Oncorhynchus mykiss) Occurs by a Respiratory Rather Than Ionoregulatory Mechanism. Aquat. Toxicol.; 63(1):65-82 http://dx.doi.org/10.1016/S0166-445X(02)00131-5